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The principal gain in imaging performance using CLSM is the ability to optically section through fluorescent objects up to 10 50 m thick or to obtain high-contrast images of surface topologies using confocal reflection optics Contrast and definition are improved, sometimes considerably, due to the reduction in background signal and a greatly improved signal-to-noise ratio With a stack of images representing different focal planes spaced at regular intervals along the optic axis (the z-axis), the object can be displayed in a variety of ways: Composite or projection view, where all of the information contained in a stack or series of images taken at a series of focal planes along the z-axis is projected into a single focused image Consider a three-dimensional object such as a fluorescent neuron with thin extended processes in a tissue section In a single wide-field fluorescence image, out-of-focus processes appear blurry and indistinct.

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A single confocal image shows the neuron extensions as fragmented streaks and dots, but a composite view of all of the focal planes contained in a stack or z-series shows the processes as continuous well-defined projections (Fig 12-7) Three-dimensional views of an object which can be obtained from a z-stack of confocal images with the help of computer software for image display Many confocal software programs can display an object at different angular perspectives or rotate the stack about the x-, y-, or z-axis, or a combination of axes, to view the cell in three dimensions This mode of viewing is valuable for examining complex threedimensional objects such as columnar epithelial cells and details in tissue sections (Fig 12-8) Transverse xz or yz cross-sectional views, which can be generated by most confocal software programs.

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Heterogeneity of machine code is resolved by registration of factories and object implementations on different hosts.

The object appears as if it had been cut transversely that is, in a plane oriented parallel to the optic axis Five-dimensional views including information in x-, y-, and z-dimensions, in a timed sequence, and in multiple colors Such sequences can be displayed as a threedimensional multicolor movie in real time or time-lapse mode Five-dimensional viewing will become more convenient as computer processing speed and storage capacity continue to improve..

RESPONSIBLE RESTRUCTURING Downsizing employees without improving efficiencies, involving workers in the decisionmaking, or determining how the cuts will better serve customers is backfiring on corporate America So said Wayne F Cascio, professor of management at the Business School at the University of Colorado at Denver, at a Society for Human Resource Management conference Cascio, who has been researching the effects of corporate downsizing for the past 15 years, sees organizations that go through a restructuring as falling into two distinct camps The first sees human capital primarily as an expense and tries to pare it down to the absolute fewest number of employees needed to run the company The second camp, which Cascio dubs the responsible restructurers, analyzes how best to utilize existing employees through redeployments and retraining Does Downsizing Really Save Money Layoffs have become a business-as-usual process for much of corporate America 1.

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96 million workers were laid off in 2002, followed by 15 million workers in 2003, according to Cascio Some organizations are even doing pre-emptive layoffs in anticipation of greater competition, such as Federal Express, which laid off thousands despite reporting record revenues and profits Essentially, there are two ways of making money: cutting costs or growing revenues, Cascio pointed out Although cost cutting is a more predictable way of boosting profits, slashing and burning human capital is a strategy that does not pay over the long haul, he said In fact, Cascio s study of S&P 500 companies found that those experiencing the greatest profitability and total return to shareholders were those that had the most stable employment base the organizations that did not lay off employees There is only one way to succeed over the long term: find ways to grow your business, Cascio concluded.

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